Substrate specificity of sheep liver sorbitol dehydrogenase

The influence of pH on the substrate specificity and stereoselectivity of alcohol dehydrogenase from horse liver.

Substrate specificity of the pyruvate dehydrogenase complex from Escherichia coli.

The investigation of the substrate specificity of the pyruvate dehydrogenase complex from Escherichia coli allows a description of the binding region of pyruvate. Substrate analogs with electronegative substitutions in the methyl group show a strong competitive inhibition of the overall reaction of the pyruvate dehydrogenase complex. The most efficient inhibitor is fluoropyruvate which has a mo...

17 -Hydroxysteroid dehydrogenase of rat skin. Substrate specificity and inhibitors.

A 17fl-hydroxysteroid dehydrogenase activity has been demonstrated in the microsomal preparations made from the skin of newborn rats. The enzyme prefers NAD to NADP as the coenzyme and utilizes 17fi-estradiol as well as testosterone as substrates. The apparent K, values estimated for 17/?-estradiol are 4.1 X 10m6 M, for estrone 1.0 X 10d5 M, and for testosterone 4.0 X 10e6 M. The enzyme can be ...

Polyol specificity of recombinant Arabidopsis thaliana sorbitol dehydrogenase studied by enzyme kinetics and in silico modeling

Polyols are enzymatically-produced plant compounds which can act as compatible solutes during periods of abiotic stress. Nicotinamide adenine dinucleotide(+)-dependent SORBITOL DEHYDROGENASE (SDH, E. C. 1.1.1.14) from Arabidopsis thaliana L. sorbitol dehydrogenase (AtSDH) is capable of oxidizing several polyols including sorbitol, ribitol, and xylitol. In the present study, enzymatic assays usi...

Some properties of aldehyde dehydrogenase from sheep liver mitochondria.

Aldehyde dehydrogenase from sheep liver mitochondria was purified to homogeneity as judged by electrophoresis on polyacrylamide gels, and by sedimentation-equilibrium experiments in the analytical ultracentrifuge. The enzyme has a molecular weight of 198000 and a subunit size of 48000, indicating that the molecule is a tetramer. Fluorescence and spectrophotometric titrations indicate that each ...